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Российские нанотехнологии. 2018; 13: 26-31

ТОКСИЧНОСТЬ ОДНОСТЕННЫХ УГЛЕРОДНЫХ НАНОТРУБОК, ИССЛЕДОВАННАЯ НА РАЗЛИЧНЫХ ТИПАХ КУЛЬТУР КЛЕТОК (ОБЗОР СОВРЕМЕННОГО СОСТОЯНИЯ ПРОБЛЕМЫ)

Тимербулатова Г. А., Фатхутдинова Л. М.

Аннотация

Одновременно с ростом глобального рынка углеродных наноматериалов, в том числе одностенных углеродных нанотрубок (ОУНТ), растет число лиц, потенциально экспонированных к аэрозолю углеродных нанотрубок. Для ускорения процесса оценки токсичности ОУНТ перспективными станут системы in vitro. За последнее десятилетие проведено изучение токсического действия ОУНТ на разных типах культур клеток, однако результаты противоречивы. В ряде исследований прослеживается дозозависимая степень проявления таких эффектов, как цитотоксичность, снижение жизнеспособности клеток, ингибирование клеточной пролиферации, апоптоз, высвобождение оксида азота, оксидативный стресс, снижение уровня антиоксидантов. Некоторые исследователи сообщили о незначительном цитотоксическом действии либо об отсутствии цитотоксичности ОУНТ на разных культурах клеток. Данные по сравнительной токсичности ОУНТ и МУНТ на сегодняшний день носят разнонаправленный характер. Одни исследователи обнаружили, что ОУНТ обладают большей цитотоксичностью, другие сообщили об отсутствии выраженных токсических эффектов для обоих типов УНТ. Оптимальный метод диспергирования ОУНТ для тестов in vitro с сохранением стабильности дисперсии остается одной из важных методологических проблем.
Список литературы

1. Transparency Market Research [Электронный ресурс]. URL: https://www.transparencymarketresearch.com/pressrelease/ carbon-nano-tubes-market.htm (дата обращения: 12.04.2018).

2. OCSiAl [Электронный ресурс]. URL: https://ocsial.com/en/ material-solutions/tuball/ (дата обращения: 12.04.2018).

3. Francis A.P., Devasena T. Toxicity of carbon nanotubes: A review // Toxicology аnd Industrial Health. 2018. V. 34. № 3. P. 200–210.

4. Liu Y., Zhao Y., Sun B., Chen C. Understanding the toxicity of carbon nanotubes // Accounts of Chemical Research. 2013. V. 46. № 3. P. 702–713.

5. Ren H., Chen X., Liu J., Gu N., Huang X. Toxicity of singlewalled carbon nanotube: How we were wrong? // Materials Today. 2010. V. 13. № 1–2. P. 6–8.

6. Hitoshi K., Katoh M., Suzuki T., Ando Y., Nadai M. Differential effects of single-walled carbon nanotubes on cell viability of human lung and pharynx carcinoma cell lines // J. Toxicol. Sci. 2011. V. 36. Р. 379–387.

7. Herzog E., Byrne H.J., Casey A., Davoren M., Lenz A.G., Maier K.L., Duschl A., Oostingh G.J. SWCNT suppress inflammatory mediator responses in human lung epithelium in vitro // Toxicol. Appl. Pharmacol. 2009 V. 234. P. 378–390.

8. Wadhwa S., Rea C., O’Hare P., Mathur A., Roy S.S., Dunlop P.S., Byrne J.A., Burke G., Meenan B., McLaughlin J.A. Comparative in vitro cytotoxicity study of carbon nanotubes and titania nanostructures on human lung epithelial cells // J. Hazard Mater. 2011. V. 191. P. 56–61.

9. Baktur R., Patel H., Kwon S. Effect of exposure conditions on SWCNT-induced inflammatory response in human alveolar epithelial cells // Toxicol. In Vitro. 2011. V. 25. Р. 1153–1160.

10. Herzog E., Casey A., Lyng F.M., Chambers G., Byrne H.J., Davoren M. A new approach to the toxicity testing of carbonbased nanomaterials—the clonogenic assay // Toxicol. Lett. 2007. V. 174. № 1–3. Р. 49–60.

11. Park E.J., Zahari N.E., Lee E.W., Song J., Lee J.H., Cho M.H., Kim J.H. SWCNTs induced autophagic cell death in human bronchial epithelial cells // Toxicol. In Vitro. 2014. V. 28. V. 3. Р.442–450.

12. Kisin E.R., Murray A.R., Keane M.J., Shi X.C., Schwegler-Berry D., Gorelik O., Arepalli S., Castranova V., Wallace W.E., Kagan V.E., Shvedova A.A. Single-walled carbon nanotubes: genoand cytotoxic effects in lung fibroblast V79 cells // J. Toxicol. Environ. Health A. 2007. V. 70. № 24. Р. 2071–2079.

13. Sharma S.C., Sarkar S., Periyakaruppan A., Barr J., Wise K., Thomas R., Wilson B.L., Ramesh G.T. Single-walled carbon nanotube induces oxidative stress in rat lung epithelial cells // J. Nanosci. Nanotechnol. 2007. V. 7. № 7. P. 2466–2472.

14. Wick P., Manser P., Limbach L.K., Dettlaff-Weglikowska U., Krumeich F., Roth S, Stark W.J., Bruinink A. The degree and kind of agglomeration affect carbon nanotube cytotoxicity // Toxicol. Lett. 2007. V. 168. P. 121–131.

15. Stoker E., Purser F., Kwon S., Park Y.B, Lee J.S. Alternative estimation of human exposure of single-walled carbon nanotubes using three-dimensional tissue-engineered human lung // Int. J. Toxicol. 2008. V. 27. № 6. Р. 441–448.

16. Davoren M., Herzog E., Casey A., Cottineau B., Chambers G., Byrne H.J., Lyng F.M. In vitro toxicity evaluation of single walled carbon nanotubes on human A549 lung cells // Toxicol. In Vitro. 2007. V. 21. № 3. P. 438–448.

17. Pulskamp K., Diabaté S., Krug H.F. Carbon nanotubes show no sign of acute toxicity but induce intracellular reactive oxygen species in dependence on contaminants // Toxicol. Lett. 2007. V. 168. Р. 58–74.

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19. Fujita K., Fukuda M., Endoh S., Kato H., Maru J., Nakamura A., Uchino K, Shinohara N., Obara S., Nagano R., Horie M., Kinugasa S., Hashimoto H., Kishimoto A. Physical properties of single-wall carbon nanotubes in cell culture and their dispersal due to alveolar epithelial cell response // Toxicol. Mech. Methods. 2013. V. 23. № 8. Р. 598–609.

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22. Luanpitpong S., Wang L., Castranova V., Rojanasakul Y. Induction of stem-like cells with malignant properties by chronic exposure of human lung epithelial cells to single-walled carbon nanotubes // Particle and Fibre Toxicology. 2014. V. 11. P. 22.

23. Wang L., Luanpitpong S., Castranova V., Tse W., Lu Y., Pongrakhananon V., Rojanasakul Y. Carbon nanotubes induce malignant transformation and tumorigenesis of human lung epithelial cells // Nano Lett. 2011. V. 11. № 7. P. 2796–2803.

24. Dumortier H., Lacotte S., Pastorin G., Marega R., Wu W., Bonifazi D., Briand J.P., Prato M., Muller S., Bianco A. Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells // Nano Lett. 2006. V. 6. № 7. P. 1522–1528.

25. Clift M.J., Endes C., Vanhecke D., Wick P., Gehr P., Schins R.P., Petri-Fink A., Rothen-Rutishauser B. A comparative study of different in vitro lung cell culture systems to assess the most beneficial tool for screening the potential adverse effects of carbon nanotubes // Toxicol Sci. 2014. V. 137. № 1. Р. 55–64.

26. Witasp E., Shvedova A.A., Kagan V.E., Fadeel B. Single-walled carbon nanotubes impair human macrophage engulfment of apoptotic cell corpses // Inhal. Toxicol. 2009. V. 21. Р. 131–136.

27. Dong P.X., Wan B., Guo L.H. In vitro toxicity of acid-functionalized single-walled carbon nanotubes: effects on murinemacrophages and gene expression profiling // Nanotoxicology. 2012. V. 6. № 3. Р. 288–303.

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42. Manna S.K., Sarkar S., Barr J., Wise K., Barrera E.V., Jejelowo O., Rice-Ficht A.C., Ramesh G.T. Single-walled carbon nanotube induces oxidative stress and activates nuclear transcription factor-kappa B in human keratinocytes // Nano Lett. 2005. V. 5. № 9. P. 1676–1684.

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54. Fujita K., Fukuda M., Endoh S., Maru J., Kato H., Nakamura A., Shinohara N., Uchino K., Honda K. Size effects of single-walled carbon nanotubes on in vivo and in vitro pulmonary toxicity // Inhalation Toxicol. 2015. V. 27. № 4. Р. 207–223.

Title in english. 2018; 13: 26-31

ТОКСИЧНОСТЬ ОДНОСТЕННЫХ УГЛЕРОДНЫХ НАНОТРУБОК, ИССЛЕДОВАННАЯ НА РАЗЛИЧНЫХ ТИПАХ КУЛЬТУР КЛЕТОК (ОБЗОР СОВРЕМЕННОГО СОСТОЯНИЯ ПРОБЛЕМЫ)

,

Abstract

Одновременно с ростом глобального рынка углеродных наноматериалов, в том числе одностенных углеродных нанотрубок (ОУНТ), растет число лиц, потенциально экспонированных к аэрозолю углеродных нанотрубок. Для ускорения процесса оценки токсичности ОУНТ перспективными станут системы in vitro. За последнее десятилетие проведено изучение токсического действия ОУНТ на разных типах культур клеток, однако результаты противоречивы. В ряде исследований прослеживается дозозависимая степень проявления таких эффектов, как цитотоксичность, снижение жизнеспособности клеток, ингибирование клеточной пролиферации, апоптоз, высвобождение оксида азота, оксидативный стресс, снижение уровня антиоксидантов. Некоторые исследователи сообщили о незначительном цитотоксическом действии либо об отсутствии цитотоксичности ОУНТ на разных культурах клеток. Данные по сравнительной токсичности ОУНТ и МУНТ на сегодняшний день носят разнонаправленный характер. Одни исследователи обнаружили, что ОУНТ обладают большей цитотоксичностью, другие сообщили об отсутствии выраженных токсических эффектов для обоих типов УНТ. Оптимальный метод диспергирования ОУНТ для тестов in vitro с сохранением стабильности дисперсии остается одной из важных методологических проблем.
References

1. Transparency Market Research [Elektronnyi resurs]. URL: https://www.transparencymarketresearch.com/pressrelease/ carbon-nano-tubes-market.htm (data obrashcheniya: 12.04.2018).

2. OCSiAl [Elektronnyi resurs]. URL: https://ocsial.com/en/ material-solutions/tuball/ (data obrashcheniya: 12.04.2018).

3. Francis A.P., Devasena T. Toxicity of carbon nanotubes: A review // Toxicology and Industrial Health. 2018. V. 34. № 3. P. 200–210.

4. Liu Y., Zhao Y., Sun B., Chen C. Understanding the toxicity of carbon nanotubes // Accounts of Chemical Research. 2013. V. 46. № 3. P. 702–713.

5. Ren H., Chen X., Liu J., Gu N., Huang X. Toxicity of singlewalled carbon nanotube: How we were wrong? // Materials Today. 2010. V. 13. № 1–2. P. 6–8.

6. Hitoshi K., Katoh M., Suzuki T., Ando Y., Nadai M. Differential effects of single-walled carbon nanotubes on cell viability of human lung and pharynx carcinoma cell lines // J. Toxicol. Sci. 2011. V. 36. R. 379–387.

7. Herzog E., Byrne H.J., Casey A., Davoren M., Lenz A.G., Maier K.L., Duschl A., Oostingh G.J. SWCNT suppress inflammatory mediator responses in human lung epithelium in vitro // Toxicol. Appl. Pharmacol. 2009 V. 234. P. 378–390.

8. Wadhwa S., Rea C., O’Hare P., Mathur A., Roy S.S., Dunlop P.S., Byrne J.A., Burke G., Meenan B., McLaughlin J.A. Comparative in vitro cytotoxicity study of carbon nanotubes and titania nanostructures on human lung epithelial cells // J. Hazard Mater. 2011. V. 191. P. 56–61.

9. Baktur R., Patel H., Kwon S. Effect of exposure conditions on SWCNT-induced inflammatory response in human alveolar epithelial cells // Toxicol. In Vitro. 2011. V. 25. R. 1153–1160.

10. Herzog E., Casey A., Lyng F.M., Chambers G., Byrne H.J., Davoren M. A new approach to the toxicity testing of carbonbased nanomaterials—the clonogenic assay // Toxicol. Lett. 2007. V. 174. № 1–3. R. 49–60.

11. Park E.J., Zahari N.E., Lee E.W., Song J., Lee J.H., Cho M.H., Kim J.H. SWCNTs induced autophagic cell death in human bronchial epithelial cells // Toxicol. In Vitro. 2014. V. 28. V. 3. R.442–450.

12. Kisin E.R., Murray A.R., Keane M.J., Shi X.C., Schwegler-Berry D., Gorelik O., Arepalli S., Castranova V., Wallace W.E., Kagan V.E., Shvedova A.A. Single-walled carbon nanotubes: genoand cytotoxic effects in lung fibroblast V79 cells // J. Toxicol. Environ. Health A. 2007. V. 70. № 24. R. 2071–2079.

13. Sharma S.C., Sarkar S., Periyakaruppan A., Barr J., Wise K., Thomas R., Wilson B.L., Ramesh G.T. Single-walled carbon nanotube induces oxidative stress in rat lung epithelial cells // J. Nanosci. Nanotechnol. 2007. V. 7. № 7. P. 2466–2472.

14. Wick P., Manser P., Limbach L.K., Dettlaff-Weglikowska U., Krumeich F., Roth S, Stark W.J., Bruinink A. The degree and kind of agglomeration affect carbon nanotube cytotoxicity // Toxicol. Lett. 2007. V. 168. P. 121–131.

15. Stoker E., Purser F., Kwon S., Park Y.B, Lee J.S. Alternative estimation of human exposure of single-walled carbon nanotubes using three-dimensional tissue-engineered human lung // Int. J. Toxicol. 2008. V. 27. № 6. R. 441–448.

16. Davoren M., Herzog E., Casey A., Cottineau B., Chambers G., Byrne H.J., Lyng F.M. In vitro toxicity evaluation of single walled carbon nanotubes on human A549 lung cells // Toxicol. In Vitro. 2007. V. 21. № 3. P. 438–448.

17. Pulskamp K., Diabaté S., Krug H.F. Carbon nanotubes show no sign of acute toxicity but induce intracellular reactive oxygen species in dependence on contaminants // Toxicol. Lett. 2007. V. 168. R. 58–74.

18. Wörle-Knirsch J.M., Pulskamp K., Krug H.F. Oops they did it again! Carbon nanotubes hoax scientists in viability assays // Nano Lett. 2006. V. 6. № 6. R. 1261–1268.

19. Fujita K., Fukuda M., Endoh S., Kato H., Maru J., Nakamura A., Uchino K, Shinohara N., Obara S., Nagano R., Horie M., Kinugasa S., Hashimoto H., Kishimoto A. Physical properties of single-wall carbon nanotubes in cell culture and their dispersal due to alveolar epithelial cell response // Toxicol. Mech. Methods. 2013. V. 23. № 8. R. 598–609.

20. Wang L., Mercer R.R., Rojanasakul Y., Qiu A., Lu Y., Scabilloni J.F., Wu N., Castranova V. Direct fibrogenic effects of dispersed single-walled carbon nanotubes on human lung fibroblasts // J. Toxicol. Environ. Health A. 2010. V. 73. № 5. R. 410–422.

21. Manke A., Luanpitpong S., Dong C., Wang L., He X., Battelli L. Derk R., Stueckle T.A., Porter D.W., Sager T., Gou H., Dinu C.Z., Wu N., Mercer R.R., Rojanasakul Y. Effect of fiber length on carbon nanotube-induced fibrogenesis // Int. J. Mol. Sci. 2014. V. 15. № 5. R. 7444–7461.

22. Luanpitpong S., Wang L., Castranova V., Rojanasakul Y. Induction of stem-like cells with malignant properties by chronic exposure of human lung epithelial cells to single-walled carbon nanotubes // Particle and Fibre Toxicology. 2014. V. 11. P. 22.

23. Wang L., Luanpitpong S., Castranova V., Tse W., Lu Y., Pongrakhananon V., Rojanasakul Y. Carbon nanotubes induce malignant transformation and tumorigenesis of human lung epithelial cells // Nano Lett. 2011. V. 11. № 7. P. 2796–2803.

24. Dumortier H., Lacotte S., Pastorin G., Marega R., Wu W., Bonifazi D., Briand J.P., Prato M., Muller S., Bianco A. Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells // Nano Lett. 2006. V. 6. № 7. P. 1522–1528.

25. Clift M.J., Endes C., Vanhecke D., Wick P., Gehr P., Schins R.P., Petri-Fink A., Rothen-Rutishauser B. A comparative study of different in vitro lung cell culture systems to assess the most beneficial tool for screening the potential adverse effects of carbon nanotubes // Toxicol Sci. 2014. V. 137. № 1. R. 55–64.

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